6c12b271-ada0-46e2-b0e1-bf1c3b707864

Robust, high-throughput, fast polarity switching quantitation of 530 mycotoxins, masked mycotoxins and other metabolites

Poisoning incidents of animals due to mycotoxin in their feed has become frequent enough to warrant the creation of regulatory requirements for detection. Protecting animal health as well as the safety of the world’s food supply is critical. Here a complete method was developed for the detection of 30 mycotoxins in animal feed using the SCIEX Triple Quad 3500 system. Using optimized sample preparation, fast chromatographic run times and a single MS method with polarity switching, quantitative results were obtained that meets regulatory requirements.

Key features of the mycotoxin workflow

  • Efficient sample preparation for animal feed, providing high extraction recovery rates
  • Easy to implement
  • Fast polarity switching: the SCIEX Triple Quad 3500 System enables the use of a single injection workflow, with both positive and negative mode acquisition to cover all 30 analytes
  • Fast run time of 13 minutes, providing high sample throughput
  • Acquisition method details provided, including chromatographic and mass spectrometry conditions for 30 mycotoxins
  • Method sensitivity that fully meets the requirements of global mycotoxin regulations for animal feed

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